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cell signaling technology ddr2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signaling technology ddr2
    shRNA-mediated knockdown of <t>DDR2.</t> ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .
    Cell Signaling Technology Ddr2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signaling+technology+ddr2/DDR2+Antibody/pmc11509142-57-4-4
    Average 94 stars, based on 65 article reviews
    cell signaling technology ddr2 - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line"

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

    Journal: Life

    doi: 10.3390/life14101260

    shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .
    Figure Legend Snippet: shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

    Techniques Used: shRNA, Knockdown, Expressing, Western Blot

    DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.
    Figure Legend Snippet: DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

    Techniques Used: Microscopy

    Related Articles

    shRNA:

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line
    Article Snippet: A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.. Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Knockdown:

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line
    Article Snippet: A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.. Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Expressing:

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line
    Article Snippet: A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.. Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Western Blot:

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line
    Article Snippet: A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.. Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Microscopy:

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line
    Article Snippet: A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.A ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA) was used for chemiluminescent detection and analysis.. Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).



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    shRNA-mediated knockdown of <t>DDR2.</t> ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .
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    shRNA-mediated knockdown of <t>DDR2.</t> ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .
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    Image Search Results


    shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

    Journal: Life

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

    doi: 10.3390/life14101260

    Figure Lengend Snippet: shRNA-mediated knockdown of DDR2. ( A ) DDR2 mRNA expression across cancer lines was ana-lyzed in the Dependency Map Portal ( https://depmap.org/portal ; Broad Institute, accessed on 15 February 2024). ( B ) Western blot of DDR2 expression (vs. GAPDH) upon 4 days of doxycycline treatment (0–12.5 mg/mL) on Tet-shDDR2 SHSY5Y cells. ( C ) Cell survival/proliferation time course assay (as measured by % confluency) of Tet-shDDR2 SHSY5Y cells treated with doxycycline (on day 0) at increasing concentrations as indicated ( n = 6 wells/condition). ( D ) Western blot of DDR2 and phospho-tyrosine 740-DDR2 (phY740-DDR2) from Tet-shDDR2 SHSY5Y cells pretreated for 3 days with the indicated concentrations of doxycycline and left unstimulated or induced with 25 mg/mL of rat-tail type I collagen for 16 h. Protein quantification data for Western blots can be found in .

    Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Techniques: shRNA, Knockdown, Expressing, Western Blot

    DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

    Journal: Life

    Article Title: Investigation of Cell Mechanics and Migration on DDR2-Expressing Neuroblastoma Cell Line

    doi: 10.3390/life14101260

    Figure Lengend Snippet: DDR2 downregulation decreases neuroblastoma cell stiffness. Young’s modulus measurements of ( A ) shCTRL and shDDR2 cells using atomic force microscopy. Indentation of ( B ) cell front (red arrow), ( C ) cell middle (blue arrow), and ( D ) cell back (yellow arrow). Young’s modulus of experiments were performed in three independent experiments. Unpaired t -test, * p < 0.05; *** p < 0.001 ( n = 32–33 cells). Data are presented as ± s.e.m.

    Article Snippet: Primary antibodies were: from Cell Signaling Technology DDR2 (#12133); from MilliporeSigma (Darmstadt, Germany) Anti-GAPDH antibody (MAB374); and from R & D Systems human phopho-DDR1/DDR2 (Y796/Y740) antibody (MAB25382).

    Techniques: Microscopy